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The importance of L1 expression in the matured brain is suggested by physiological and behavioral studies showing that L1 is related to hippocampal plasticity and fear conditioning.

The distribution of L1 in mouse brain might provide a basis for understanding its role in the brain.

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We examined the overall distribution of L1 in the adult mouse brain by immunohistochemistry using two polyclonal antibodies against different epitopes for L1.

Immunoreactive L1 was widely but unevenly distributed from the olfactory bulb to the upper cervical cord.

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High to moderate density of neuronal L1 was found in the olfactory bulb, layer V of the cerebral cortex, amygdala, pontine grey, superior colliculi, cerebellar cortex, solitary tract nucleus etc.

Only low to lowest levels of neuronal L1 were found in the hippocampus, grey matter in the caudate-putamen, thalamus, cerebellar nuclei etc. In the adult brain, substantial immunoreactive L1 is detected by western blot and immunohistochemical analyses in the olfactory bulb, cerebellum, cerebral cortex, hippocampus, hypothalamus and spinal cord [].

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